With Avian Support, Engineered Cells Turn into Drug Factories
To encode ncAAs that have distinct chemical, biological and physical properties, bioorthogonal translational machines are required. These consist of a pair of evolved aminoacyl-tRNA synetase/tRNA and a blank codon. The researchers achieved this by mimicking the ability of the ibis to synthesize and incorporate sTyr into proteins.
The Xiao laboratory used a mutant stop codon amber to encode the desired sulfotransferase. This resulted in a mammalian cell-line that was capable of biosynthesizing sTyr with high precision and incorporating it into proteins.
The authors of the study wrote that these engineered cells can produce \”site specific sulfated protein at a greater yield than cells fed with the highest levels of sTyr in the literature.\” These cells were used to prepare highly effective thrombin inhibitors with site specific sulfation.